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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GOMES, L. M. C.; CASTRO, A. V.; DIAS, L. O.; GOMES, F. P.; ALMEIDA, A-A. F.; PIROVANI, C. P.; GESTEIRA, A. da S. |
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Afiliação: |
Luana Mahé Costa Gomes, UESC; A. V. CASTRO, UESC; L. O. DIAS, UESC; Fábio Pinto Gomes, UESC; Alex-Alan Furtado Almeida, UESC; Carlos Priminho Pirovani, UESC; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Cadmium-induced changes in leaf protein profile of Caesalpinia peltophoroides Benth (sibipiruna): protein extraction and bidimentional eletrophoresis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos... [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
pdf 34634 |
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Conteúdo: |
The specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in Milli-Q water until the appearance of spots. The gel images were captured in 2D Scanner Magic and analyzed with the software ImageMaster 2D Platinum (Amersham Biosciences) and manual detection. In 2D-PAGE spots were visualized 99 spots (41 differentially expressed) in the 24 h and 122 spots (29 differentially expressed) in the 72 h, both with quality. Most intense spots were analyzed in the Expasy Tagident (www.expasy.ch/), based on its mass and isoelectric point. Taxonomic group and keywords were used to search in the protein database. Preliminary results identified proteins such as: aquaporins, heat shock (HSP), cold and drought-regulated protein (CORA), photosystem I assembly, NAD(P)H-quinone oxidoreductase subunit and cytochrome b6-f complex subunit in Populus, Arabidopsis and Pisum sativum. Some spots were not found in the database. Spots will be selected for the protein sequencing by mass spectrometry. This is a pioneering study involving proteomic data in order to support the hypothesis that C. peltophoroides can be considered a promising species in tolerance to Cd stress. MenosThe specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in M... Mostrar Tudo |
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Palavras-Chave: |
Environmental pollution; Proteomic. |
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Thesaurus Nal: |
abiotic stress; heavy metals; phytoremediation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03475nam a2200253 a 4500
001 1886916
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aGOMES, L. M. C.
245 $aCadmium-induced changes in leaf protein profile of Caesalpinia peltophoroides Benth (sibipiruna)$bprotein extraction and bidimentional eletrophoresis.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos... [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34634
520 $aThe specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in Milli-Q water until the appearance of spots. The gel images were captured in 2D Scanner Magic and analyzed with the software ImageMaster 2D Platinum (Amersham Biosciences) and manual detection. In 2D-PAGE spots were visualized 99 spots (41 differentially expressed) in the 24 h and 122 spots (29 differentially expressed) in the 72 h, both with quality. Most intense spots were analyzed in the Expasy Tagident (www.expasy.ch/), based on its mass and isoelectric point. Taxonomic group and keywords were used to search in the protein database. Preliminary results identified proteins such as: aquaporins, heat shock (HSP), cold and drought-regulated protein (CORA), photosystem I assembly, NAD(P)H-quinone oxidoreductase subunit and cytochrome b6-f complex subunit in Populus, Arabidopsis and Pisum sativum. Some spots were not found in the database. Spots will be selected for the protein sequencing by mass spectrometry. This is a pioneering study involving proteomic data in order to support the hypothesis that C. peltophoroides can be considered a promising species in tolerance to Cd stress.
650 $aabiotic stress
650 $aheavy metals
650 $aphytoremediation
653 $aEnvironmental pollution
653 $aProteomic
700 1 $aCASTRO, A. V.
700 1 $aDIAS, L. O.
700 1 $aGOMES, F. P.
700 1 $aALMEIDA, A-A. F.
700 1 $aPIROVANI, C. P.
700 1 $aGESTEIRA, A. da S.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 30 | |
| 6. |  | GOMES, F. P.; GOMES, L. M. C.; LAVIOLA, B. G.; GRIFFITHS, H. Water relations and carbon isotope composition in young plants of two provenances of Jatropha curcas L. subjected to water deficit. In: CONGRESSO DA REDE BRASILEIRA DE TECNOLOGIA DE BIODIESEL, 6.; CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 9., 2016, Natal, RN. Biodiesel: 10 anos de pesquisa, desenvolvimento e inovação no Brasil: anais. Lavras: UFLA, 2016. Não paginado.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | MELO, W. F. de; GOMES, L. M.; MOITA, A. W.; AMARO, G. B.; BESSA, F. P. de; DUSI, A. N. Biofortificação no Brasil (BioFort): Avaliação preliminar de clones de batata-doce ricos em betacaroteno. Horticultura Brasileira, Brasília, DF, v. 29, n. 2, p. 2675-2680, jul. 2011. CD-ROM. Suplemento. Trabalho apresentado no 51. Congresso Brasileiro de Olericultura, Viçosa, MG.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | RIBEIRO, C. S. da C.; SOARES, R. S.; GOMES, L. M.; COELHO, L. G. F.; REIFSCHNEIDER, F. J. B. Breeding Calabrian pepper lines for Brazilian agriculture from sui generis introduction of germplasm. Horticultura Brasileira, Brasília, DF, v. 35, n. 2, p. 195-202, apr./june 2017. 0102-0536| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 9. | | RIBEIRO, C. S. da C.; SOARES, R. S.; GOMES, L. M.; COELHO, L. G. F.; REIFSCHNEIDER, F. J. B. Breeding calabrian pepper lines (capsicum annuum L.) for Brazilian agriculture from sui generis introduction of germplasm. In: EUCARPIA CAPSICUM AND EGGPLANT WORKING GROUP MEETING, 16., Kecskemét, 2016. Proceedings of XVI EUCARPIA capsicum and eggplant working meeting in memoriam Dr. Alain Palloix. Budapest: Diamond Congress Ltd., 2016. p. 313-320. Editado por Katalin Ertsey-Peregi, Zsuzsanna Füstös, Gábor Palotás e Gábor Csilléry.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | RAGASSI, C. F.; ZUCOLOTTO, J.; GOMES, L. M.; RIBEIRO, C. S. da C.; MADEIRA, N. R.; REIFSCHNEIDER, F. J. B. Productivity, quality of fruits and architecture of Jalapeño pepper at different planting densities. Horticultura Brasileira, v. 37, n. 3, p. 331-337, Jul./Sept. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 12. | | BRITO, B. G.; KAWAICHI, M. E.; PINHEIRO, A. R.; GOMES, L. M.; JAENISCH, F. R. F.; TAGLIARI, K. C. Ocorrência de Salmonella sp. e Mycoplasma sp. em pombos domésticos. In: CONFERÊNCIA APINCO DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 2007, Santos, SP. Anais... Campinas: FACTA, 2007. Trabalhos de pesquisa. p. 220. Projeto n. 01.02.10.406-04.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 14. | | MELO, W. F. de; SILVA, J. B. C. da; GOMES, L. M.; MOITA, A. W.; AMARO, G. B.; FERNANDES, F. R.; NUTTI, M. R.; DUSI, A. N. Biofortificação no Brasil (BioFORT): avaliação preliminar de clones de batata-doce ricos em betacaroteno em duas épocas de plantio. In: REUNIÃO DE BIOFORTIFICAÇÃO NO BRASIL, 4., 2011, Teresina. Palestras e resumos... Rio de Janeiro: Embrapa Agroindústria de Alimentos; Teresina: Embrapa Meio-Norte, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Hortaliças. |
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| 15. | | SILVA, E. M. da; GOMES, L. M.; TORGA, P. P.; VIANELLO, R. P.; SOUZA, T. L. P. O. de; RESENDE, M. P. M. Análise de associação genômica ampla (GWAS) dos grupos comerciais do feijão-comum. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 13., 2021, Goiânia. Conectividade tecnológica, intensificação sustentável: resumos. Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2021. p. 137. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 16. | | SILVA, E. M. da; GOMES, L. M.; TORGA, P. P.; VIANELLO, R. P.; SOUZA, T. L. P. O. de; RESENDE, M. P. M. Análise de associação genômica ampla (GWAS) do hábito de crescimento em feijoeiro-comum. In: SEMINÁRIO JOVENS TALENTOS, 14., 2020, Santo Antônio de Goiás. Resumos... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2021. p. 64.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 17. | | OLIVEIRA, D. V. V.; COELHO, L. G. F.; GOMES, L. M.; RIBEIRO, C. S. da C.; MOITA, A. W.; REIFSCHNEIDER, F. J. B. Produtividade de linhagens avançadas e produção de semente genética de pimenta do tipo Jalapeño Amarelo. Horticultura Brasileira, Brasília, DF, v. 31, n. 2, p. S3644-S3651, jul. 2014. Suplemento. Trabalho apresentado no 53. Congresso Brasileiro de Olericultura, jul. 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 18. | | CARVALHO, S. I. C. de; RIBEIRO, C. S. da C.; BIANCHETTI, L. de B.; GOMES, L. M.; OLIVEIRA, D. V. V.; REIFSCHNEIDER, F. J. B. Processo de proteção da cultivar de pimenta BRS Juruti. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | CARVALHO, S. I. C. de; RIBEIRO, C. S. da C.; BIANCHETTI, L. de B.; GOMES, L. M.; OLIVEIRA, D. V. V.; REIFSCHNEIDER, F. J. B. Processo de proteção da cultivar de pimenta BRS Juruti. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 30 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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